cAMP Stimulates Transepithelial Short-Circuit Current and Fluid Transport Across Porcine Ciliary Epithelium
نویسندگان
چکیده
منابع مشابه
Fluid transport across the isolated porcine ciliary epithelium.
PURPOSE To quantify spontaneous fluid transport across the isolated porcine ciliary epithelium and determine its sensitivity to the electrolyte transport inhibitors ouabain and bumetanide, as well as bath Cl(-) and HCO(3)(-) levels. METHODS A complete annulus of ciliary body was mounted in a custom-designed chamber appropriate for quantifying net fluid movement, as well as the transepithelial...
متن کاملcAMP inhibits transepithelial chloride secretion across bovine ciliary body/epithelium.
PURPOSE To investigate the potential significance of cAMP in the regulation of Cl(-) transport across the bovine ciliary body/epithelium (CBE). METHODS Fresh native bovine CBE preparation was mounted in a modified Ussing chamber. The effects of cAMP-stimulating agents on short-circuit current (I(sc)) and net (36)Cl(-) secretion were determined. RESULTS Addition of cAMP-stimulating agents in...
متن کاملRole of anions in nitric oxide-induced short-circuit current increase in isolated porcine ciliary processes.
PURPOSE To investigate how nitric oxide (NO) modulates short-circuit current (Isc) in isolated porcine ciliary processes. METHODS Isc changes (Ussing-type chamber) induced either by the NO donors SNP or SIN-1, or by the cGMP analogue 8-pCPT-cGMP were assessed. The effect of inhibitors of guanylate cyclase (10 microM ODQ, 100 microM LY83583), protein kinase G (30 microM Rp-8-pCPT-cGMP, 3 micro...
متن کاملActive transport of ascorbate across the isolated rabbit ciliary epithelium.
The transepithelial transport of ascorbate across the isolated rabbit ciliary epithelium (CE) was investigated. Unidirectional 14C-ascorbate fluxes were measured in the presence of equal concentrations of ascorbate on both sides of the tissue within the range of 0.025 to 1 mM. The blood to aqueous (Bl----Aq) flux increased from 6 to 95 nmoles/hr and showed nonlinearity and saturation. The aqueo...
متن کاملEpinephrine stimulates fluid absorption across bovine retinal pigment epithelium.
The authors used a modified capacitive probe technique to simultaneously assess the effect of apical epinephrine on fluid transport rate (Jv), transepithelial potential (TEP), and transepithelial resistance (Rt) across bovine retinal pigment epithelium (RPE). In control Ringer, the RPE absorbed fluid at a rate of 1.42 +/- 0.34 microliters/cm2.hr (mean +/- SEM; 22 tissues). Tissues with the high...
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ژورنال
عنوان ژورنال: Investigative Opthalmology & Visual Science
سال: 2016
ISSN: 1552-5783
DOI: 10.1167/iovs.16-20127